Vescovi fa ricerca sana e che dà frutti. Sono d'accordo col ministro Turco.
Vescovi fa ricerca sana e che dà frutti. Sono d'accordo col ministro Turco.
A me la ricerca sulle staminali embrionali non dà alcun problema etico; e noi non abbiamo mai sostenuto che si dovesse fare solo la ricerca sulle staminali embrionali, bensì che si dovesse fare anche quel tipo di ricerca.
Che infatti si fa ovunque nel mondo, tranne che in Italia.
bisogna vedere seguire la via "etica" quanto ci costa
e quanto ci è costato trovare questa strada e dove saremmo arrivati se facevamo altrimenti......
Manca il link al post di apertura del thread. O arriva il link o cancello il thread.
Allora ho letto e questo è l' abstract .
Comment in:
Nature. 2007 Jul 19;448(7151):260-2.
Generation of germline-competent induced pluripotent stem cells.
Okita K, Ichisaka T, Yamanaka S.
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
We have previously shown that pluripotent stem cells can be induced from mouse fibroblasts by retroviral introduction of Oct3/4 (also called Pou5f1), Sox2, c-Myc and Klf4, and subsequent selection for Fbx15 (also called Fbxo15) expression. These induced pluripotent stem (iPS) cells (hereafter called Fbx15 iPS cells) are similar to embryonic stem (ES) cells in morphology, proliferation and teratoma formation; however, they are different with regards to gene expression and DNA methylation patterns, and fail to produce adult chimaeras. Here we show that selection for Nanog expression results in germline-competent iPS cells with increased ES-cell-like gene expression and DNA methylation patterns compared with Fbx15 iPS cells. The four transgenes (Oct3/4, Sox2, c-myc and Klf4) were strongly silenced in Nanog iPS cells. We obtained adult chimaeras from seven Nanog iPS cell clones, with one clone being transmitted through the germ line to the next generation. Approximately 20% of the offspring developed tumours attributable to reactivation of the c-myc transgene. Thus, iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
PMID: 17554338 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/sites/en...ubmed_RVDocSum
Related article
Comment in:
Nature. 2007 Jul 19;448(7151):260-2.
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K, Bernstein BE, Jaenisch R.
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Nuclear transplantation can reprogramme a somatic genome back into an embryonic epigenetic state, and the reprogrammed nucleus can create a cloned animal or produce pluripotent embryonic stem cells. One potential use of the nuclear cloning approach is the derivation of 'customized' embryonic stem (ES) cells for patient-specific cell treatment, but technical and ethical considerations impede the therapeutic application of this technology. Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene expression and chromatin state of such induced reprogrammed stem cells are similar to those of ES cells. Notably, the cells-derived from mouse fibroblasts-can form viable chimaeras, can contribute to the germ line and can generate live late-term embryos when injected into tetraploid blastocysts. Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.
PMID: 17554336 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/sites/en...RVAbstractPlus